human chronic lymphocytic leukemia cell line jvm 3  (DSMZ)

Journal: NPJ genomic medicine

Article Title: PanCancer analysis of somatic mutations in repetitive regions reveals recurrent mutations in snRNA U2.

doi: 10.1038/s41525-022-00292-2

Figure Lengend Snippet: Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from CLL JVM3 cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.

Article Snippet: CLL cell line JVM3 (DSMZ) was grown in RPMI 1640, 10% FBS, 1% PSG and 1% non-essential amino acids.

Techniques: Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Control, Western Blot, Sequencing

Journal: Cells

Article Title: Progression and Differentiation of Alveolar Rhabdomyosarcoma Is Regulated by PAX7 Transcription Factor—Significance of Tumor Subclones

doi: 10.3390/cells10081870

Figure Lengend Snippet: Transcription factors regulating myogenic differentiation were differentially expressed in ERMS and ARMS cells. mRNA expression levels were evaluated with qPCR. Data were calculated with 2 − ΔCt method using GAPDH as a constitutive gene. The data on graphs show means ± SEM, n = 3–4. Statistical analysis was performed with ANNOVA with Tukey posttest to analyze differences between ERMS and ARMS cell lines. Two types of differences were shown. * symbol indicates statistically significant differences compared to RD ERMS cell lines ( p < 0.05), whereas $ symbol designates statistically significant differences compared to RH18 ERMS cell line ( p < 0.05).

Article Snippet: RMS cell lines—RH30, RH41, and RD—were kindly provided by Dr. PJ Houghton (Center for Childhood Cancer, Columbus, OH, USA), additionally RH30 cell line was ordered from ATCC (American Type Culture Collection, Manassas, VA, USA), and RH18 cell line from DSMZ (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures in Germany).

Techniques: Expressing

Journal: Cells

Article Title: Progression and Differentiation of Alveolar Rhabdomyosarcoma Is Regulated by PAX7 Transcription Factor—Significance of Tumor Subclones

doi: 10.3390/cells10081870

Figure Lengend Snippet: Myogenic microRNAs were differentially expressed in ERMS and ARMS cells. miR-1-3p, miR-133a-3p, miR-133b, and miR-206 expression levels were calculated with 2 − ΔCt method using miR-103a-3p as a constitutive gene. The data on graphs show means ± SEM, n = 3–4. Statistical analysis was performed with ANNOVA with Tukey posttest to analyze differences between ERMS and ARMS cell lines. Two types of differences were shown. * symbol indicates statistically significant differences compared to RD ERMS cell lines ( p < 0.05), whereas $ symbol designates statistically significant differences compared to RH18 ERMS cell line ( p < 0.05).

Article Snippet: RMS cell lines—RH30, RH41, and RD—were kindly provided by Dr. PJ Houghton (Center for Childhood Cancer, Columbus, OH, USA), additionally RH30 cell line was ordered from ATCC (American Type Culture Collection, Manassas, VA, USA), and RH18 cell line from DSMZ (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures in Germany).

Techniques: Expressing